Influence of paraquat on oxidative stress of dopaminergic neuron in substantia nigra of mice / 上海第二医科大学学报

ABSTRACT

Objective To explore the oxidative stress pathogenesis of Parkinson's disease(PD) induced by paraquat in substantia nigra of mice. Methods The model of PD was established by oral administration of paraquat to mice.The spectrophotometry was used to determine the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-PX) and the content of malondialdehyde(MDA) in substantia nigra.At the same time,number of tyrosine hydroxylase(TH) positive neurons in substantia nigra of mice was estimated by immunohistochemistry. Results The activities of SOD and GSH-PX were significantly decreased,and the content of MDA was increased in paraquat-treated mice compared to that of mice treated by saline taken orally(P