Effect of Age on Proliferation of Rat Vascular Smooth Muscle Cells Stimulated with Fetal Bovine Serum, Angiotensin II and Phorbol 12-Myristate 13-Acetate / 대한해부학회지

ABSTRACT

The present study investigated effects of fetal bovine serum (FBS), angiotensin II (Ang II) and phorbol 12 -myristate 13 -acetate (PMA) on proliferation of vascular smooth muscle cells (VSMC) isolated from aorta of 5, 9, 12 and 17 week old Sprague -Dawley rats. With 10% FBS, proliferation rate of VSMCs was markedly greater in 17 week old group than in 5 week old group. Treatment of 0.1 ~10 micro M Ang II or 10 and 100 micro M PMA in 0.1% bovine serum albumin and 10% FBS media did not influence the cell proliferation. However, in 5% FBS media, Ang II and PMA enhanced the proliferation of the VSMC from all age groups except 5 week. To test the effects of Ang II in the FBS, captopril, an Ang converting enzyme inhibitor, and ibersartan, an Ang II receptor antagonist, were pretreated. Neither 10 micro M captopril nor ibersartan in 5% FBS influenced the VSMC proliferation. These results indicate that the VSMCs from older rats proliferate faster than those from 5 week -old rats, and 5% FBS -induced proliferation was enhanced by Ang II and PMA.