Local Injection of Granulocyte-Colony Stimulating Factor Accelerates Wound Healing in a Rat Excisional Wound Model
Tissue Engineering and Regenerative Medicine
; (6): 297-303, 2016.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-649673
Responsible library:
WPRO
ABSTRACT
A systemic treatment of granulocyte-colony stimulating factor (G-CSF) is known to improve healings of damaged tissues. However, recent studies suggested local actions of G-CSF on the healing processes of damaged tissues. We investigated the treatment effect of locally injected G-CSF and compared to that of systemically injected G-CSF in a rat model. A wound was created on the rat dorsum and treated either by local injection or by systemic injection of G-CSF. Wound healing rate, deposition of collagen, and gene expression were evaluated. G-CSF receptor (G-CSFR) protein was detected by Western blotting. The wound healing rate in the local injection group was significantly higher than that in the systemic injection group at days 9 and 15; it was also significantly higher than that in the control group at days 3, 9, and 15. The expression of G-CSFR protein in wound tissues was higher than in normal skin tissues. The local injection of G-CSF is more effective than systemic injection of G-CSF in promoting wound healing, which may implicate the local action of G-CSF treatment in wound healing processes.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Skin
/
Wound Healing
/
Wounds and Injuries
/
Gene Expression
/
Blotting, Western
/
Granulocyte Colony-Stimulating Factor
/
Collagen
/
Receptors, Granulocyte Colony-Stimulating Factor
/
Models, Animal
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Tissue Engineering and Regenerative Medicine
Year:
2016
Document type:
Article