Effect of Copper on the Regulation of Ferroportin-1 Gene Expression
The Korean Journal of Nutrition
; : 434-441, 2009.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-649771
Responsible library:
WPRO
ABSTRACT
Ferroportin-1 (FPN) is a transporter protein that is known to mediate iron export from macrophages. The purpose of this study was to investigate the effect of copper on the regulation of FPN gene expression in J774 mouse macrophage cells. J774 cells were treated with various concentrations of CuSO4 and RT-PCR analyses were performed to measure the steady-state levels of mRNAs for FPN and divalent metal transporter 1 (DMT1, an iron importer). Copper treatment significantly increased FPN mRNAs in a dose-dependent manner, but didn't change the levels of DMT1 mRNA. Experiments with transcriptional inhibitor actinomycin D (0.5 microgram/mL) revealed that copper treatment did not affect the half-life of FPN mRNAs in J774 cells. On the other hand, results from luciferase reporter assays showed that copper directly stimulated the promoter activity of FPN. In summary, our data showed copper induced FPN mRNA of macrophages via a transcriptional rather than post-transcriptional mechanisms.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Gene Expression
/
Copper
/
Dactinomycin
/
Half-Life
/
Hand
/
Iron
/
Luciferases
/
Macrophages
Limits:
Animals
Language:
Korean
Journal:
The Korean Journal of Nutrition
Year:
2009
Document type:
Article