The Morphological Study of the Protein Gene Product (PGP) 9.5-like Immunoreactive Enteric Nervous System in Murine Ileum: Close Relation to Interstitial Cells of Cajal (ICC) in Confocal Laser Scanning Microscopy / 대한해부학회지
Korean Journal of Anatomy
; : 153-159, 2002.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-651768
Responsible library:
WPRO
ABSTRACT
This study was performed to investigate the morphology of the enteric nervous system and interstitial cells of Cajal (ICC) in the murine ileum. The PGP9.5-like immunoreactive (PGP9.5-LI) neurons and the c-Kit-like immunoreactive (c-Kit-LI) ICCs were stained by indirect immunofluorescence method and were observed under the confocal laser scanning microscopy. According to three dimensional reconstruction study, it was found that the PGP9.5-LI neurons and the c-Kit-LI ICCs were widely distributed in the intestinal wall (1) In circular muscle layer, PGP9.5-LI nerve fibers were paralell to circular muscle layer. (2) In the myenteric plexus, the PGP9.5-LI nerve were closely apposed to the adjacent PGP9.5-LI nerve, constituting the networks. (3) In double-labeling immunohistochemistry using anti-PGP9.5 and anti-c-Kit antibodies, the c-Kit-LI networks encircled around the neural strands. The characteristic arrangement of the PGP9.5-LI enteric nervous system and the ICC containing c-Kit-positive cells provide a morphological basis upon the mechanism regulating gastro-intestinal motility and the pathogenesis of gatro-intestinal disorders.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Immunohistochemistry
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Enteric Nervous System
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Microscopy, Confocal
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Fluorescent Antibody Technique, Indirect
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Interstitial Cells of Cajal
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Ileum
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Antibodies
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Myenteric Plexus
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Nerve Fibers
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Neurons
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Anatomy
Year:
2002
Document type:
Article