Optimization of Recombinant Human Platelet-Derived Growth Factor-BB Encapsulated in Poly (Lactic-co-Glycolic Acid) Microspheres for Applications in Wound Healing
Tissue Engineering and Regenerative Medicine
; (6): 13-20, 2016.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-654717
Responsible library:
WPRO
ABSTRACT
Growth factors play multiple and critical roles in wound repair processes. Platelet-derived growth factor (PDGF) is a potent growth factor that is particularly important in the early inflammatory phase of wound healing. In order to extend the half-life of PDGF, polymeric encapsulation is used. In the current study, Poly (lactic-co-glycolic acid) (PLGA) microspheres containing recombinant human (rh) PDGF-BB were prepared to prolong the effectiveness of this growth factor. PLGA microspheres were optimized using a modified w/o/w-double-emulsion/solvent evaporation method by changing the processing conditions of stirring speed and emulsifier (polyvinyl alcohol) concentration. Microspheres prepared using the optimized method released rhPDGF-BB for up to three weeks. An in vitro migration assay showed a significant decrease in the wound area in cells treated with rhPDGF-BB microspheres compared to control cells. These findings demonstrate the potential of rhPDGF-BB encapsulated in microspheres to enhance wound healing.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Polymers
/
Wound Healing
/
Wounds and Injuries
/
In Vitro Techniques
/
Platelet-Derived Growth Factor
/
Intercellular Signaling Peptides and Proteins
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Half-Life
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Methods
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Microspheres
Limits:
Humans
Language:
English
Journal:
Tissue Engineering and Regenerative Medicine
Year:
2016
Document type:
Article