TGF - beta Type II Receptor Anomaly and of Its Functional Restoration in Osteosarcoma Cell Lines / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
; : 914-919, 1998.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-656673
Responsible library:
WPRO
ABSTRACT
TGF-p receptor mutation is now considered as one of the carcinogenic process of many tumors. To evaluate whether there is an abnormality in TGF-p type II receptor in osteosarcoma cell lines, we performed Northern analysis, cross-linking assay, luciferase activity and TGF-p growth inhibition assay in four osteosarcoma cell lines G292, U202, HOS and SaOS. We also transfected the tumor cells with normal TGF-p type II receptor sequence to find if there is a possibility of gene therapy in osteosarcoma. In Northern analysis, Type II receptor expressions were decreased at SaOS, U202 and HOS cell lines. In cross-linking assay, all four cell lines didnt show type II receptor at their cell surface. The growth of these tumor cells were not suppressed by TGF-p. From these findings, we concluded that the normal production of TGF-p type II receptor was impaired in osteosarcoma. The transfection of these tumor cells with normal type II receptor sequence restored growth inhibition by TGF-p. This means even though TGF-p type II receptor is abnormal in osteosarcoma, we can restore its function by transfection of normal sequence. We think that the TGF-p type Il receptor gene therapy can be one of the treatment method for osteosarcoma in the future.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Genetic Therapy
/
Osteosarcoma
/
Cell Line
/
Luciferases
Language:
Korean
Journal:
The Journal of the Korean Orthopaedic Association
Year:
1998
Document type:
Article