Simultaneous Quantitative Determination of Five Saponins in Notoginseng Total Saponins by HPLC with a Monolithic Column / 中国药师
China Pharmacist
; (12): 1879-1881, 2017.
Article
in Zh
| WPRIM
| ID: wpr-658113
Responsible library:
WPRO
ABSTRACT
Objective:To establish a rapid HPLC method for the quantitative determination of 5 saponins ( notoginsenoside R1 , ginsenoside Rg1 ,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd) in Notoginseng total saponins using a monolithic column. Meth-ods:The analysis was performed on a Merck Chromolith Performance RP-18e column (100 mm × 4. 6 mm,2μm) with gradient elution of acetonitrile and water. The detection wavelength was set at 203 nm. Results:Satisfactory separation of all analytes was obtained in 20 min. All calibration curves showed good linearity within the testing ranges (r≥0.9998). The average recoveries were between 98. 6% and 100. 4%. The RSDs were less than 2. 1% (n=6). Conclusion:The method is efficient and accurate for the quality con-trol of Notoginseng total saponins.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
China Pharmacist
Year:
2017
Document type:
Article