Composition and clinical significance of γδ T lymphocytes in peripheral blood of Gestational Diabetes Mellitus patients / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 2497-2499, 2017.
Article
in Zh
| WPRIM
| ID: wpr-658388
Responsible library:
WPRO
ABSTRACT
Objective To compare the composition of γδ T cells in the peripheral blood of gestational diabetes mellitus(GDM) patients with those of normal pregnant women,so as to explore the association of γδ T cells with the onset and progression of GDM.Methods The peripheral blood mononuclear cells(PBMC) of GDM patients(case group) and normal pregnant women(con-trol group) were separated by Ficoll-Hypaque density gradient centrifugation.γδ T cells were labeled with FITC conjugated anti TCRγδ monoclonal antibody(McAb) and PE conjugated anti-CD3 McAb and analyzed by flow cytometry.Results γδ T cells in GDM patients' peripheral blood were about(6.89 ± 3.22) %,which was higher than normal control group(4.26 ± 1.64) %.The percentage of γδ T cells in non-treated GDM group was significantly increased to(8.79 ± 2.33)%,while in treated GDM group,the percentage of γδ T cells was drop to normal(3.76±1.62)%.Conclusion γδ T cells in GDM patients' peripheral blood and CD3+ T Cells were slightly increased.The alteration may be related to the onset of GDM.
Full text:
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Database:
WPRIM
Language:
Zh
Journal:
International Journal of Laboratory Medicine
Year:
2017
Document type:
Article