Effects of β-carotene on expression of cell tight junction protein of IPEC-J2 with LPS-stimulated / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 1611-1615, 2017.
Article
in Zh
| WPRIM
| ID: wpr-667644
Responsible library:
WPRO
ABSTRACT
Objective:To investigate the mechanism of β-carotene anti-inflammatory on intestinal epithelial cells.Methods:The piglet jejunum epithelium(IPEC-J2)cell line was used as an cell model.The cells were divided into 4 groups[control group,β-carotene group,β-carotene pre-protective group and Lipopoly-saccharide(LPS)group].The control group was not treated,β-carotene group and β-carotene pre-protective group were pretreated with β-carotene.Lipopoly-saccharide(LPS)and β-carotene pre-protective groups were stimulated with LPS.The cell viability was detected by MTT.Western blot was performed to detect the expression of Occludin,Claudin4 and ZO-1 tight junction proteins.Results:The expression of IPEC-J2 cell tight junction protein in LPS group were significantly lower than that of the control group(P<0.05).The expression of tight junction protein in β-carotene pre-protective group was significantly higher than that in LPS group(P<0.05).Conclusion:Increasing the expression of tight junction proteins may be one of the ways that anti-inflammatory effect of β-carotene in jejunum epithelial cells.
Full text:
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Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Immunology
Year:
2017
Document type:
Article