The effects of enamel matrix proteins on the synthesis of alkaline phosphatase and type I collagen in osteoblasts / 实用口腔医学杂志
Journal of Practical Stomatology
; (6)1995.
Article
in Zh
| WPRIM
| ID: wpr-670589
Responsible library:
WPRO
ABSTRACT
Objective:To study the effects of enamel matrix proteins (EMPs) on the biological characters of osteoblasts.Methods:MC3T3-E1 osteoblasts were cultured in vitro and exposed to EMPs at 300,400 and 500 ?g/ml.Enzyme kinetic method was used to assay alkaline phosphatase (ALP) synthesis and 3H-proline labelling combining collagenase digestion method was used to assay type I collagen synthesis.Results:The results manifested that ALP level in EMPs treated groups was higher than that in control group (P
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Journal of Practical Stomatology
Year:
1995
Document type:
Article