Isolation and Preparation of Phloroglucinol Derivatives from Dryopteris Fragrans by Semi-preparation HPLC / 中国药师
China Pharmacist
; (12): 545-547,548, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-671139
Responsible library:
WPRO
ABSTRACT
Objective:
To isolate and prepare the reference substances of phloroglucinol derivatives from Dryopteris fragrans by semi-preparation HPLC.Methods:
After reflux extraction of Dryopteris fragrans with petroleum ether,the extracting solution was con-centrated and separated by silica gel column chromatography,and then isolated by semi-preparation HPLC. The isocratic elution was carried out using acetonitrile as the mobile phase at 3. 0 ml·min-1 and the injection volume was 0. 5 ml. Two phloroglucinol deriva-tives were isolated.Results:
The chemical structure of the two phloroglucinol derivatives respectively was aspidin BB with the purity of 98. 81% and aspidin PB with the purity of 98. 57% by ultra high performance liquid chromatography.Conclusion:
The isolation of as-pidin BB and aspidin PB by semi-preparation HPLC is simple and fast with the purity over 98%,which can be used to prepare the ref-erence substances.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2015
Document type:
Article