Effective of Basic Fibroblast Growth Factor on Fetal Rat Brain Damage / 中华围产医学杂志

ABSTRACT

Objective To investigate if basic fibroblast Growth factor (bFGF) can penetrate placental barrier, alleviate rat brain damage and stimulate fetal rat neurons proliferation. Methods BFGF labeled with 125 I was injected peritoneally to pregnant rats, and tissue distribution of radioactivity of 125 I in different organs was detected. Thirty two of pregnant Waister rats were randomly divided at 15 d of gestational age into four groups normal control, uteri distressed control, bFGF treatment, and bFGF prevention. Fetal rats in the latter three groups suffered from distress in uteri in an animal model of perinatal asphyxia. Proliferated neurons in fetal rat brains were counted in each group. Results 125 I bFGF has been found in fetal brain, heart, lung and spleen, etc. Under high power microscope, proliferated neurons in fetal rat brains were counted in each group, they are (4.5 ?2.4), (5.8?3.1), (17.2?5.4); (18.1?5.8), ( F=128,P