Improvement of MTT assay by 2-chloroadenosine in activation test and cytotoxicity test of lymphocyte / 中国免疫学杂志
Chinese Journal of Immunology
; (12)2000.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-674953
Responsible library:
WPRO
ABSTRACT
Objective:
To sutdy the effect of 2 chloroadenosine(2 ClA),which is specifically cytotoxic to macrophages,on MTT assay in activation test and cytotoxicity test of lymphocyte.Methods:
Using cell culture technique,mouse splenic lymphocytes and peritoneal macrophages were cultured.Lymphocyte activation and specific cytotoxicity to tumor cells and toxicity of 2 ClA to macrophages were measured by MTT assay in the presence or absence of 2 ClA.Results:
2 ClA had a strong cytotoxic effect on macrophages.When the activation test and cytotoxicity test of lymphocyte were measured by MTT assay,the optical density values of 2 ClA group was lower than that of control group,and statistic analysis showed P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2000
Document type:
Article