Study of methods of PCR-ELISA in testing the RNA of platelet activating factor receptor / 中国免疫学杂志
Chinese Journal of Immunology
; (12)1985.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-675422
Responsible library:
WPRO
ABSTRACT
Objective:
To study the methods of PCR ELISA in testing the RNA of platelet activating factor receptor.Methods:
Extracting the total RNA of 20 normal people and 20 psoriasis patients,the PAF R and ? actin RNA assayed by RT PCR,PAF R sense labelled by Biotin and antisense labelled by Digoxin,? actin sense labelled by Biotin and antisense labelled by fluorescein.The results of PCR were tested by ELISA and Agar gel electrophoresisa.Results:
The positive number of PAF R RNA was 16 by Agar gel electrophoresis and 18 by PCR ELISA in 20 normal people;The positive number of PAF R RNA was 18 by Agar gel electrophoresisa and 20 by PCR ELISA in 20 Psoriasis patients;The positive number of ? actin was 20 in two groups with two methods.Conclusion:
The sense and antisense labelled by different substance in PCR ELISA has high sensitivity and the course was simple in testing the PAF R RNA.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1985
Document type:
Article