Identifying difference in mRNA expression of prostate epithelial cells cocultured with and without stromal cells / 中华泌尿外科杂志

ABSTRACT

Objective To identify the difference in mRNA expression of prostate epithelial cells cocultured with and without stromal cells. Methods Cocultured model and DDRT PCR were used to analyze different mRNA expression of prostate epithelial cells with and without stromal cells.The different expression sequence tags (ESTs) were obtained,then identified by reverse dot hybridization,and sequenced.The obtained cDNA sequence was compared with that in the public database. Results It was found that there was a significant difference of gene expression in prostate epithelial cells.70 differential display cDNA fragments were obtained.After they were sequenced,45 ESTs were found to share high homology to the known genes,whereas the remaining 25 ones were novel. Conclusions There is different gene expression in prostate epithelial cells cocultured with and without stromal cells.These differential genes may play a role in the interaction between epithelial cells and stromal cells.