Establishment and evaluation of animal tumor model expressing HER2/neu / 第三军医大学学报
Journal of Third Military Medical University
; (24)2003.
Article
in Zh
| WPRIM
| ID: wpr-678406
Responsible library:
WPRO
ABSTRACT
Objective To establish animal tumor model expressing human oncogene HER2/neu and to evaluate the model by immunological method. Methods The recombinant eukaryotic plasmid expressing HER2/neu gene was transfected into P815 tumor cells. The tumor cell line P815 expressing HER2/neu gene stably was screened by limiting dilution method. The expression of target gene in host cells was determined by Western blotting. Following the establishment of animal tumor model expressing HER2 antigen in DBA/2 mice, tumor specific response was induced in the spleen cells of DBA/2 mice which were inoculated with recombinant plasmids. Results P815 cell line expressing HER2/neu oncogene stably was obtained. HER2/neu specific CTLs could be induced from immunized DBA/2 mice. Conclusion HER2/neu expressing tumor animal model is established successfully and the in vivo immune response can be induced after immunization with HER2/neu genetic vaccine.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Journal of Third Military Medical University
Year:
2003
Document type:
Article