Diagnostic value of transbronchial lung biopsy combined with immunohistochemistry in pulmonary interstitial fibrosis and the clinical significance / 第三军医大学学报

ABSTRACT

Objective To evaluate the diagnostic value in pulmonary interstitial fibrosis by transbronchial lung biopsy(TBLB) and immunohistochemistry. Methods The lung tissue samples were obtained from 20 cases of pulmonary fibrosis due to different causes, including 9 cases of idiopathic pulmonary fibrosis(IPF) and 11 cases of pulmonary fibrosis caused by chronic obstructive pulmonary disease (PF COPD), by means of transbronchial lung biopsy. Hematoxylin and eosin staining, Masson trichrome staining and immunostaining with anti type Ⅲ collagen and anti type I collagen multiclonal antibodies were performed. Differences in clinical characteristics, pathological manifestations, levels, types and distribution of proliferation of collagen fibers between IPF and PF COPD were compared. Results The proliferation of collageneous fibers was significantly higher in IPF than in PF COPD( P