Effects of NDGA on the growth of a human malignant glioma cell line CHG-5 / 第三军医大学学报
Journal of Third Military Medical University
; (24)2003.
Article
in Zh
| WPRIM
| ID: wpr-678914
Responsible library:
WPRO
ABSTRACT
Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the growth of a human malignant glioma cell line CHG 5 in vitro and in vivo . Methods Colorimetric MTT assay, flow cytometry, and light microscopy were used to investigate the proliferation in vitro , cell cycles, apoptosis of CHG 5 cells, and the growth of xenografted tumor in nude mice. Results NDGA significantly inhibited the proliferation of CHG 5 cells in vitro . Cells in G 0/G 1 phase increased, but cells in S, G 2/M phases decreased, and apoptotic cells increased significantly. After treatment of NDGA (50 mg/kg, intraperitoneally) at 5 d after the inoculation of tumor cells, the xenografted tumor volume reduced remarkably without causing significant toxic and side effects. Conclusion The inhibitory effect of NDGA on the growth of CHG 5 cells may be correlated with the regulation of cell cycles and induction of apoptosis.
Full text:
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Database:
WPRIM
Language:
Zh
Journal:
Journal of Third Military Medical University
Year:
2003
Document type:
Article