Effect of Protein and Anthraquinone Glycosides from Radix Polygoni Multiflori on Learning and Memory and Metabolites in D-galactose-induced Aging Mice / 广州中医药大学学报

ABSTRACT

Objective To observe the effects of protein and anthraquinones glycosides from Radix Polygoni Multiflori(RPM) on learning and memory as well as associated enzymes and metabolites in mice with D-galactose-induced aging.Methods Eighty Kunming mice were equally randomized into 8 groups normal group,model group,vitamin E(90mg?kg-1?d-1) group,high-and low-dose RPM protein(20 and5 mg?kg-1?d-1 respectively) groups,high-and low-dose RPM anthraquinone glycoside(28 and 7mg?kg-1?d-1 respectively) groups,combination(low-dose RPM protein and low-dose RPM anthraquinone glycosides)group.Except the normal group,the mice in other groups received subcutaneous injection of D-galactose to indue the aging model.The learning rate of mice was detected with trisection radiation maze,memory was evaluated by one-time passive avoidance response,cerebral superoxide dismustase(SOD) and monoamine oxidase(MAO) activities as well as malondialdehyde(MDA)content were detected with ultraviolet spectrophotometer,and hepatic lipofuscin(LF) content was examined with fluorescence spectrophotometer.Results Memory of aged mice was much improved in high-dose RPM protein group(P