Cloning and Sequencing of Cytochrome c Oxidase II(COII) Gene of Three Species of Mosquitoes / 中国寄生虫学与寄生虫病杂志

ABSTRACT

Objective To determine and compare the complete sequence of COII gene from Anopheles sinensis, Aedes albopictus and Culex quinquefasciatus and to identify the molecular homology among these mosquitoes. Methods The recombinant plasmids including COII gene from the three species of mosquitoes were obtained by the T A cloning technique,and the COII genes were sequenced and analyzed. Results The homology of the COII gene nucleotide sequence and the deduced amino acid sequence of COII among the three species of mosquitoes varied from 84.1% to 87.9% and from 85.1% to 89.5%, respectively. The C+G content of COII gene was 23.2%-24.9%, the frequency of transversion higher than that of transition. Conclusion Aedes albopictus and Culex quinquefasciatus are closely related species at their COII molecular level according to COII gene.