Effect of intrahepatic cholestasis on development and growth of fetus in pregnant rats / 医学研究生学报

ABSTRACT

Objectives:To observe the effect of intrahepatic cholestasis on the development and growth of fetus in pregnant rats. Methods: The animal models of intrahepatic cholestasis were made by injecting benzogynestryl (5 mg/kg body weight) or cholate (2.4 mg/kg body weight) for 5 days starting from 16th day of pregnancy. Biochemical parameters of fetal plasma and amniocent were determined at the 21st day of pregnancy. The pathological lesion of placenta in rats was also observed. Results: Cholic acid group showed lower body weight and high level of estrodiol in fetal plasma with high metaphase mortality of fetus. The level of TBA in amniocent was high in estrodiol group compared with normal control group. Pathologically, placenta edema and chorion denaturalization and necrosis with infiltration of neutrocyte were observed in both groups. Conclusions: Intrahepatic cholestasis is detrimental to the development and growth of fetal rats in pregnancy rats.