Study on difference of inflammatory cytokines and STAT3 expressions in intestinal mucosal tissue between Uyghur and Han patients with ulcerative colitis in Xinjiang region / 重庆医学

ABSTRACT

Objective To explore the difference of inflammatory cytokines and STAT3 expressions in intestinal mucosal tissue between Uyghur and Han patients with ulcerative colitis (UC) in Xinjiang Uyghur Autonomous Region.Methods Thirty inpatients (15 Uyghur cases and 15 Han cases) with UC in this hospital from June 2015 to October 2016 were selected as the UC group.Contemporaneous 26 individuals (13 Uyghur cases and 13 Han cases) undergoing physical examination served as the control group.The colonic mucosa tissue were taken for detecting the mRNA expression of IFN-γ,IL-22,IL-17A and IL-17F by reversal transcription-polymerase chain reaction (RT-PCR).The expression of STAT3 was detected by using the immunohistochemical staining technique.Results Compared with the Uyghur control group,the mRNA levels of IFN-γ,IL-22,IL-17A and IL-17F in the Uyghur UC group were up-regulated,the differences were statistically significant (P＜0.05);compared with the Han healthy control group,the mRNA levels of above inflammatory cytokines in the Han UC group were up-regulated,the differences were statistically significant (P＜0.05);but the mRNA levels of above inflammatory cytokines had no statistical difference between the Uyghur UC group and Han UC group (P＞0.05).The immunohistochemical method found that the STAT3 was found to be colored in the cytoplasm of intestinal mucosal epithelial cells.The positive expression rate of STAT3 in Han and Uyghur UC groups was higher than that in the corresponding control group,the differences were statistically significant (P＜0.05),however there was no statistically significant difference between Uyghur UC group and Han UC group,the difference was not statistically significant (P＞0.05).Conclusion In Xinjiang Uyghur Autonomous Region,the expressions of intestinal mucosal IFN-γ,IL-22,IL-17A,IL-17F and STAT3 in Uyghur and Han patients with UC are significantly increased,but the above cytokines may has no obvious difference in UC occurrence and development between Uyghur and Han patients.