Distinction of Cells Infected with Respiratory Syncytial Virus by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry / 分析化学

ABSTRACT

Respiratory syncytial virus (RSV),which is a major cause of lower respiratory tract infections during infancy and childhood,is also an important pathogen for immunosuppression in elder and diseased areas.In order to research the pathogenesis of RSV,the matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS)was used to explore the difference between uninfected cells and virus-infected cells.The result showed that,in the molecular weight range of 5000~10000 Da,there are three component peaks expressed with significant difference in both normal group and infection group.One of the three components was up-regulated (m/z 6154.25) and the other two were down-regulated (m/z 7658.47 and 9259.82) after RSV infection.This result proved the obvious differences between the infected cells and untreated cells.The differential expression may provide a feasible method for RSV disease diagnosis and pathology research,and also provide scientific evidences for research on development of RSV therapeutic drugs.