Hepatocytes apoptosis induced by β1-adrenoceptor autoantibody is involved in hepatic dysfunction / 基础医学与临床
Basic & Clinical Medicine
; (12): 626-631, 2018.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-693954
Responsible library:
WPRO
ABSTRACT
Objective To investigate the effect of β1-Adrenoceptor autoantibody on liver function.Methods The biologically active of β1-AA was prepared and passive immunization model was established with β1-AA.The bio-chemical parameters of the liver were measured by the automatic serum biochemical analyzer.The liver size, hepatic vein,portal vein velocity were detected by liver ultrasound;hepatocytes apoptosis were tested by tunel stai-ning,annexin V/PI staining and caspase 3 activity detection.Results The biologically active of β1-AA and passive immunization model were established successfully.The ALT and AST of the liver significantly increased and the ALB decreased in the passive immunization process.The apoptosis of the hepatocytes increased,and meto-prolol partially reversed this effect.Conclusions β1-AA may induce hepatocytes apoptosis by β1-adrenergic receptor and participate in the development of liver injury.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Basic & Clinical Medicine
Year:
2018
Document type:
Article