Development of a 30 Y-STR Loci with middle or low mutations multiplex PCR system / 中国法医学杂志
Chinese Journal of Forensic Medicine
; (6): 11-16, 2018.
Article
in Zh
| WPRIM
| ID: wpr-701473
Responsible library:
WPRO
ABSTRACT
Objective To deveplope construct and validate a novel multiplex PCR system comprised of 30 Y-STR markers only with low and moderate mutation rates. Methods 30 Y-STRs characterized by low/moderate mutation rate and middle/high polymorphic was amplified simultaneously in a multiplex PCR system using the six color labeling fluorescence. PCR product was analyzed in a ABI 3500XL Genetic Analyzer. The accuracy, specifity, sensitivity and stability of the system and its validation on the mixtures were evaluated. Results The validation studies demonstrated that the system is a stable, accurate, and sensitive multiplex PCR system. The sensitivity was 0.0625ng DNA. Y-STR could be detection in a male/female DNA mixture ratio of 1:4. Conclusion The primary study demonstrates that this multiplex PCR system is effective and reliable for forensic routine DNA analysis. It will be very helpful for constructing Chinese forensic Y-STR database and population genetic research.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Forensic Medicine
Year:
2018
Document type:
Article