Interactions between induced T regulatory cells and B cells in inflammatory milieu in mice with collagen-induced arthritis / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 815-819,825, 2018.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-702823
Responsible library:
WPRO
ABSTRACT
Objective:
To investigate the interactions between induced T regulatory cells ( iTregs ) and B cells in the inflammatory milieu in mice with collagen-induced arthritis(CIA).Methods:
CD19+ cells were isolated from the spleen cells of normal DBA1/J(N-B) mice and CIA mice(CIA-B) on the 35th day after the first immunization with established arthritis. These B cells were used as antigen-presenting cells to observe their effects on the induction of Tregs. Tregs were induced with the classic method and co-cultured with CIA-B cells. CIA-B cell effects on iTreg proliferation and the expression of CTLA-4 on iTregs were explored. iTregs′ influence on the expressions of co-stimulators(CD80,CD86) and MHCⅡon B cells was studied and its mechanism was determined by the Transwell experiments.Results:
CIA-B could induce more Treg production and proliferation. CIA-B could also promote the CTLA-4 expression on iTreg cell surface which worked through a cell-contact pathway, while iTregs could increase the expressions of co-stimulators(CD80,CD86) and MHCⅡby the same way.Conclusion:
iTregs could show their immune suppressive function through the interactions with CIA-B cells in the inflammatory milieu in mice with CIA. These interactions work by a cell-contact pathway.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2018
Document type:
Article