Effect of acteoside on dysfunction of learning and memory and oxidative stress in rats with vascular dementia / 中华行为医学与脑科学杂志

ABSTRACT

Objective To explore the effects of acteoside on dysfunction of learning and memory and the protective effect of oxidative stress in rats with vascular dementia.Methods 30 SD rats were randomly divided into sham group,model group,nicergoline group,low-dose acteoside group and high-dose acteoside group,with 6 rats in each group.Preparation of vascular dementia model by 2-vessed occlusion.The ability of exploring and learning and memory in rats were detected by step down test and avoid dark test.Determination of malondialdehyde (MDA),reactive oxygen species (ROS) and glutathione peroxidase (GSH-PX) activity in serum and brain tissue was conducted by Elisa.Results Autonomic activity test showed that the frequency and activity of autonomous activity in the model group were significantly lower than those in the sham group(P＜0.01),the frequency of autonomous activity in each administration group was significantly higher than that in the model group (P＜0.01),and the central activity time in the high-dose group was significantly higher than that in the model group (P＜0.01).Step down test and avoid dark test showed that the latency of the model group was significantly lower than that of the sham group.(Model group of step down test:(25.33 ± 3.01) s,Sham group of step down test:(56.83 ± 15.90)) (P＜ 0.01).(Model group of avoid dark test:(15.67 ± 3.61) s,Sham group of avoid dark test:(135.82±44.00) s) (P＜0.01).The latency of low dose group was significantly higher than that of model group.(Low dose group of step down test:(46.40±14.32) s) (P＜0.01).(Low dose group of avoid dark test (44.20± 8.26)s) (P＜0.05).Step down test and avoid dark test showed that the number of mistakes in the model group was significantly higher than that in the sham operation group(P＜0.01).The number of errors in nicergoline group and the low dose group was significantly lower than that in the model group (P＜0.01,P＜0.05).In serum,the content of MDA and ROS in model group was significantly higher than that in sham group (P＜0.01) while the activity of GSH-PX in model group was significantly lower than that of sham group.The content of MDA in the other groups was significantly lower than that in the model group (P＜0.01).The content of ROS in the nicergoline group and low dose group was significantly lower than that in the model group (P＜0.05,P＜0.0l).The activity of GSH-PX in high dose group was significantly higher than that in model group (P＜0.01).In brain tissue,the content of MDA and Ros in model group was significantly higher than that in sham group (P＜0.01).The content of MDA and ROS in low dose group and high dose group were significantly lower than that in model group (P＜0.05,P＜0.01).Conclusion Acteoside can improve the dysfunction of learning and memory and depressive mood disorder caused by vascular dementia and reduce oxidative stress injury by decreasing the content of MDA-ROS and increasing the activity of GSH-PX enzyme.