lmmunogenicity of HER2 vaccine containing p-nitrophenylalanine / 中国药科大学学报
Journal of China Pharmaceutical University
; (6): 369-375, 2018.
Article
in Zh
| WPRIM
| ID: wpr-704348
Responsible library:
WPRO
ABSTRACT
In order to delete the immune tolerance of self-protein HER2 and produce an efficient immune response,the genetic code expansion technique was employed to introduce the immunogenic amino acid p-nitro-phenylalanine into the position 5 ,26 and 79 of the HER2 fragment and HER2 mutants containing p-nitropheny-lalanine were obtained.Prototype and mutant HER2 molecules with a purity of more than 95% were obtained through a nickel affinity column.Immunogenicity analysis of the HER2 vaccine showed that pNO2Phe79HER2 was able to produce high titer cross-reactive antibodies in C57BL/6 mice and that the mouse antisera could recognize SKBR-3 cells with intact HER2 receptor extracellular domain and cleave HER2 highly expressed HER2 +B16F10 cells through ADCC effect.These findings suggested that pNO2Phe79HER2 which incorporates p-nitro-phenylalanine could be used as a tumor vaccine candidate targeting HER2.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Journal of China Pharmaceutical University
Year:
2018
Document type:
Article