Expression and purification of human placental growth factor 2 using the Pichia pastoris expression system / 中国药科大学学报
Journal of China Pharmaceutical University
; (6): 376-380, 2018.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-704349
Responsible library:
WPRO
ABSTRACT
To prepare the human placental growth factor 2 (PIGF-2 ),human PIGF-2 gene was cloned into pPIC9K vector to construct the recombinant pPIC9K-PIGF-2 vector. Linearized recombinant pPIC9K-PIGF-2 was transformed into Pichia pastoris by electroporation. YPD-Geneticin plate was used to screen geneticin hyper-resistant colonies. The positive colonies were verified by PCR. Results of SDS-PAGE and Western blot showed that recom-binant human PIGF-2 was expressed after being induced by methanol. Using its characteristic heparin binding, recombinant human PIGF-2 was successfully purified by heparin affinity column chromatography.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of China Pharmaceutical University
Year:
2018
Document type:
Article