Patterns of rpoC Mutations in Drug-Resistant Mycobacterium tuberculosis Isolated from Patients in South Korea / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
; : 222-227, 2018.
Article
in En
| WPRIM
| ID: wpr-715740
Responsible library:
WPRO
ABSTRACT
BACKGROUND: Rifampicin (RFP) is one of the principal first-line drugs used in combination chemotherapies against Mycobacterium tuberculosis, and its use has greatly shortened the duration of chemotherapy for the successful treatment of drug-susceptible tuberculosis. Compensatory mutations have been identified in rpoC that restore the fitness of RFP-resistant M. tuberculosis strains with mutations in rpoB. To investigate rpoC mutation patterns, we analyzed 93 clinical M. tuberculosis isolates from patients in South Korea. METHODS: Drug-resistant mycobacterial isolates were cultured to determine their susceptibility to anti-tubercular agents. Mutations in rpoC were identified by sequencing and compared with the relevant wild-type DNA sequence. RESULTS: In total, 93 M. tuberculosis clinical isolates were successfully cultured and tested for drug susceptibilities. They included 75 drug-resistant tuberculosis species, of which 66 were RFP-resistant strains. rpoC mutations were found in 24 of the 66 RFP-resistant isolates (36.4%). Fifteen different types of mutations, including single mutations (22/24, 91.7%) and multiple mutations (2/24, 8.3%), were identified, and 12 of these mutations are reported for the first time in this study. The most frequent mutation involved a substitution at codon 452 (nt 1356) resulting in amino acid change F452L. CONCLUSION: Fifteen different types of mutations were identified and were predominantly single-nucleotide substitutions (91.7%). Mutations were found only in dual isoniazid- and RFP-resistant isolates of M. tuberculosis. No mutations were identified in any of the drug-susceptible strains.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Rifampin
/
Tuberculosis
/
Codon
/
Base Sequence
/
Tuberculosis, Multidrug-Resistant
/
Drug Resistance, Multiple
/
Drug Therapy
/
Drug Therapy, Combination
/
Korea
/
Mycobacterium
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Tuberculosis and Respiratory Diseases
Year:
2018
Document type:
Article