Anti-inflammatory activities of Scolopendra subspinipes mutilans in RAW 264.7 cells / 한국영양학회지
Journal of Nutrition and Health
; : 323-329, 2018.
Article
in Ko
| WPRIM
| ID: wpr-716207
Responsible library:
WPRO
ABSTRACT
PURPOSE: The dried body of Scolopendra subspinipes mutilans has long been used as a traditional Korean medicinal food, but little is known about its mechanisms of action. In this study, we investigated the anti-inflammatory activities of Scolopendra subspinipes mutilans and possible mechanisms in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. METHODS: Cytotoxicity of Scolopendra subspinipes mutilans extract (SSME) was measured by MTT assay, anti-inflammatory activities were analyzed by nitric oxide (NO) production, the expression of inducible NO synthase (iNOS) and the mRNA level of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6). Nuclear translocation of nuclear factor-kappa B (NF-κB) p65 subunit and degradation of inhibitory kappa B (IκB) were examined by western blot. RESULTS: SSME inhibited LPS-induced NO production and iNOS expression without cytotoxicity. Up-regulation of LPS-induced pro-inflammatory cytokines, IL-1β and IL-6 was dose dependently attenuated by SSME. Exposure of pyrrolidine dithiocarbamate, an NF-κB specific inhibitor, accelerated the inhibitory effects of SSME on NO production and iNOS expression in LPS-stimulated cells. Moreover, translocation of NF-κB from the cytosol to the nucleus and degradation of IκB were decreased by treatment with SSME in LPS-induced cells. CONCLUSION: These results suggest that the SSME might have the inhibitory effects on inflammation, partly through inhibition of the NF-κB signaling pathway.
Key words
Full text:
1
Database:
WPRIM
Main subject:
RNA, Messenger
/
Up-Regulation
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Blotting, Western
/
Cytokines
/
Interleukin-6
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Nitric Oxide Synthase
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Cytosol
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Inflammation
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Nitric Oxide
Language:
Ko
Journal:
Journal of Nutrition and Health
Year:
2018
Document type:
Article