Lineage-specific chimerism analysis in nucleated cells, T cells and natural killer cells after myeloablative allogeneic hematopoietic stem cell transplantation
Korean Journal of Hematology
; : 18-23, 2011.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-720643
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Chimerism analysis is an important tool for assessing the origin of hematopoietic cells after allogeneic stem cell transplantation (allo-SCT) and can be used to detect impending graft rejection and the recurrence of underlying malignant or nonmalignant diseases.METHODS:
This study included 24 patients who underwent myeloablative allo-SCT. DNA was extracted from nucleated cells (NCs), T cells, and natural killer (NK) cells, and the chimerism status of these cell fractions was determined by STR-PCR performed using an automated fluorescent DNA analyzer.RESULTS:
Twenty-three out of the 24 patients achieved engraftment. Mixed chimerism (MC) in NCs, but not in T cells and NK cells, was significantly correlated with disease relapse. MC in all cell fractions was correlated with mortality. Ten patients (41.6%) developed extensive chronic GVHD. Six patients had MC in T cells, and 3 of them had chronic GVHD. Four patients with MC and relapse received donor lymphocyte infusion (DLI), and among them, 3 had secondary relapse. Further, the chimerism status differed among different cell lineages in 6 patients with myeloid malignancies.CONCLUSION:
The implications of MC in lymphocyte subsets are an important area for future research. Chimerism analysis in lineage-specific cells permits detection of relapse and facilitates the monitoring of therapeutic interventions. These results can provide the basic data for chimerism analysis after myeloablative SCT.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Recurrence
/
Tissue Donors
/
DNA
/
Hematopoietic Stem Cells
/
Killer Cells, Natural
/
Lymphocytes
/
T-Lymphocytes
/
Lymphocyte Subsets
/
Hematopoietic Stem Cell Transplantation
/
Cell Lineage
Limits:
Humans
Language:
English
Journal:
Korean Journal of Hematology
Year:
2011
Document type:
Article