PLC-gamma1 for Differentiating Adenocarcinoma from Reactive Mesothelial Cells in Effusions / 대한세포병리학회지
Korean Journal of Cytopathology
; : 115-119, 1997.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-726388
Responsible library:
WPRO
ABSTRACT
Cytologic diagnosis of reactive or malignant effusion is sometimes difficult. Espe- cially, differentiation of benign reactive mesothelial cells from malignant cells in body effusion is more difficult. Recently, immunohistochemistry has been used to diagnose difficult cases. Phospholipase C(PLC)-gamma 1 is one of the isoenzyme of the PLC which plays central role in signal transduction involving cellular growth, differentiation and transformation by phosphorylating many protein component. Increased expression of PLC-gamma 1 in human breast carcinoma, colorectal carcinoma and stomach cancers are reported. To evaluate the efficacy of positive PLC-gamma 1 immunostaining in the diagnosis of malignancy in effusions, paraffin-embedded cell blocks of pleural fluid and ascites from 10 patients(5 metastatic adenocarcinomas, and 5 reactive mesothelial cells) were immunostained with a monoclonal antibody to PLC-gamma 1. PLC-gamma 1 immuostained all the adenocarcinomas in cell block(5/5) with intense membrane pattern, however, none of the reactive mesothelial proliferations stained with the diagnostic membrane pattern. Thus, our study strongly supports the conclusion that PLC-gamma 1 immunopositivity is likely to become a useful adjunct for the diagnosis of malignancy in effusions.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Phospholipases
/
Ascites
/
Stomach Neoplasms
/
Breast Neoplasms
/
Immunohistochemistry
/
Colorectal Neoplasms
/
Adenocarcinoma
/
Signal Transduction
/
Diagnosis
/
Phospholipase C gamma
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Cytopathology
Year:
1997
Document type:
Article