Development of Estimation Methods of Skin Oxidation and Evaluation of Anti-Oxidative Effects of Genistein in Topical Formulations
The Korean Journal of Physiology and Pharmacology
; : 205-209, 2012.
Article
in En
| WPRIM
| ID: wpr-728099
Responsible library:
WPRO
ABSTRACT
The objective of the present study was to establish the method of measurement of hydrogen peroxide and to estimate the anti-oxidative effect of genistein in the skin. UVB induced skin oxidation and anti-oxidative effect of genistein formulations were evaluated by determining levels of hydrogen peroxide. The mechanism involved in the determination of hydrogen peroxide is based on a color reaction between ferric ion (Fe3+) and xylenol orange, often called FOX assay and subsequent monitoring of absorbance values of the reactant at 540 nm. The reaction was to some extent pH-dependent and detection sensitivity was greatest at pH 1.75. Genistein liposomal gel demonstrated better anti-oxidative effect with regard to lowering hydrogen peroxide levels elevated by UVB irradiation compared to genistein-suspended gel. A linear relationship has been observed between anti-oxidative effect of genistein and drug deposition in the skin tissue. Genistein liposomal gel resulting in the localization of the drug in the deeper skin led to improved anti-oxidative effect compared to genistein gel. The suggested method for evaluation of oxidation of the skin can be used as a tool to screen effective anti-oxidative agents and their delivery systems acting on the skin.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Phenols
/
Skin
/
Sulfoxides
/
Genistein
/
Citrus sinensis
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Hydrogen-Ion Concentration
/
Hydrogen Peroxide
Language:
En
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2012
Document type:
Article