Effects of TGF-beta1 Ribbon Antisense on CCl4-induced Liver Fibrosis
The Korean Journal of Physiology and Pharmacology
; : 1-6, 2008.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-728196
Responsible library:
WPRO
ABSTRACT
Ribbon-type antisense oligonucleotide to TGF-beta1(TGF-beta1 RiAS) was designed and tested to prevent or resolve the fibrotic changes induced by CCl4 injection. When Hepa1c1c7 cells were transfected with TGF-beta1 RiAS, the level of TGF-beta1 mRNA was effectively reduced. TGF-beta1 RiAS, mismatched RiAS, and normal saline were each injected to mice via tail veins. When examined for the biochemical effects on the liver, TGF-beta1 mRNA levels were significantly reduced only in the TGF-beta1 RiAS-treated group. The results of immunohistochemical studies showed that TGF-beta1 RiAS prevented the accumulation of collagen and alpha-smooth muscle actin, but could not resolve established fibrosis. These results indicate that ribbon antisense to TGF-beta1 with efficient uptake can effectively prevent fibrosis of the liver.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Veins
/
Fibrosis
/
RNA, Messenger
/
Actins
/
Collagen
/
Transforming Growth Factor beta1
/
Liver
/
Liver Cirrhosis
/
Muscles
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Physiology and Pharmacology
Year:
2008
Document type:
Article