A simple method of separating outer hair cells in Guinea pig / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 829-831, 2008.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-746541
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#In order to meet the needs of electrophysiology outer hair cells, we investigate a method to separate outer hair cells simply and effectively.@*METHOD@#The basal membrane was dissected combined with spiral ligament and axis of cochlea, then incubated with enzyme, and then mechanically triturated by micropipette.@*RESULT@#A large number of living outer hair cells were obtained. Of 80% cells could keep good condition in 6 hours.@*CONCLUSION@#If increasing the enzyme concentration slightly and the large part of cochlea without outer bone was enzymes, we can get living outer hair cells easily.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Cell Separation
/
Cochlea
/
Cell Biology
/
Guinea Pigs
/
Hair Cells, Auditory
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2008
Document type:
Article