Effects of sodium valproate on proliferation and apoptosis of Hep-2 cells and associated mechanism / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 359-362, 2010.
Article
in Zh
| WPRIM
| ID: wpr-747997
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To observe the effect of sodium valproate (VPA) on proliferation and apoptosis of human laryngeal carcinoma Hep-2 cells and its associated mechanism.@*METHOD@#Methabenzthiazuron (MTT) was used to observe the proliferation of human laryngeal carcinoma Hep-2 cells treated with various concentrations of VPA at different times. Flow cytometry(FCM) and RT-PCR were used to measure the apoptosis rate and the expression of Survivin mRNA in the Hep-2 cells treated with VPA at 3 mmol/L for different times.@*RESULT@#VPA inhibited growth of Hep-2 cells in a dose-dependent and time-dependent manner (P < 0.01). The apoptosis rate increased after the treatment by VPA at 3 mmol/L. There were significant differences between different time groups (P < 0.01). The expression of Survivin mRNA of Hep-2 cells were decreased in a time dependent manner (3 mmol/L) (P < 0.01).@*CONCLUSION@#VPA have obvious growth inhibition and induction of apoptosis on human laryngeal cancer Hep-2 cells, its mechanism is related to decrease the expression of Survivin.
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Valproic Acid
/
Apoptosis
/
Cell Line, Tumor
/
Cell Proliferation
/
Inhibitor of Apoptosis Proteins
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Flow Cytometry
/
Survivin
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Year:
2010
Document type:
Article