Determination of ginsenoside Rg1 and Re in Tongru granules by HPLC / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
; (6): 497-501, 2019.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-751752
Responsible library:
WPRO
ABSTRACT
Objective To establish a rapid and efficient method for the simultaneous determination of ginsenoside Rg1 and ginsenoside Re in Tongru granules by HPLC.Methods The HPLC analysis was carried out with Agilent ZORBAX SB-C18 and acetonitrile-0.1% phosphoric acid(1585,V/V) as mobile phase,the flow rate was 1.0 ml/min,the column temperature was 30 ℃,injection volume was 10 μl,and the detection wavelength was 203 nm.Results The Rg1 solution of ginsenoside showed a good linear relation between 0.136-2.040 μg (r2=0.999 9).The ginsenoside Re solution presented a good linear relationship (r2=0.999 8)between 0.066-0.990 μg,with the average addition recovery of 99.3% and 99.1%,and the relative standard deviation of 0.84% and 0.75%(n=6),respectively.Conclusions The method is highly specific,sensitive,reproducible,simple and efficient which can be used for quality control of Tongru Granules.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Journal of Traditional Chinese Medicine
Year:
2019
Document type:
Article