The mutation of mitochondrial genome in lymphoma / 肿瘤研究与临床

ABSTRACT

Objective To investigate the mutation of mitochondrial genome in lymphoma. Methods The peripheral blood or borrow fluid 2 ml from 14 lymphoma patients in the First Hospital of Qinhuangdao between May 2016 and July 2017 were collected. Polymerase chain reaction (PCR) was used to amplify and sequence mitochondrial DNA, and the results were compared with the revised Cambridge reference sequence (rCRS) and human mitochondrial genome database (mtDB), and then the mutation was also analyzed. Results There were 118 mutation genes, including 57.63％ (68/118) in D-loop region, 18.64％ (22/118) in NADH dehydrogenase 5 (ND5) region, 13.56％(16/118) in cytochrome b oxidase (CbO) region, 5.08％(6/118) in ND1 region, 3.39％ (4/118) in cytochrome oxidase (COⅡ) region, 1.69％ (2/118) in ND4 region. Conclusion Mitochondrial DNA mutation in lymphoma has a high mutation rate.