The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection
Protein & Cell
; (12): 590-600, 2017.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-756983
Responsible library:
WPRO
ABSTRACT
Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. However, the exact binding site of EV71 on SCARB2 is unknown. In this study, we generated a monoclonal antibody (mAb) that binds to human but not mouse SCARB2. It is named JL2, and it can effectively inhibit EV71 infection of target cells. Using a set of chimeras of human and mouse SCARB2, we identified that the region containing residues 77-113 of human SCARB2 contributes significantly to JL2 binding. The structure of the SCARB2-JL2 complex revealed that JL2 binds to the apical region of SCARB2 involving α-helices 2, 5, and 14. Our results provide new insights into the potential binding sites for EV71 on SCARB2 and the molecular mechanism of EV71 entry.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Receptors, Virus
/
Thermodynamics
/
Virology
/
Binding Sites
/
Recombinant Fusion Proteins
/
Immunoglobulin Fab Fragments
/
Models, Molecular
/
Gene Expression
/
Cell Line
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Protein & Cell
Year:
2017
Document type:
Article