Colonic Transit Disorder Mediated by Downregulation of Interstitial Cells of Cajal/Anoctamin-1 in Dextran Sodium Sulfate-induced Colitis Mice
Journal of Neurogastroenterology and Motility
; : 316-331, 2019.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-765928
Responsible library:
WPRO
ABSTRACT
BACKGROUND/AIMS:
Interstitial cells of Cajal (ICC) and their special calcium-activated chloride channel, anoctamin-1 (ANO1) play pivotal roles in regulating colonic transit. This study is designed to investigate the role of ICC and the ANO1 channel in colonic transit disorder in dextran sodium sulfate (DSS)-treated colitis mice.METHODS:
Colonic transit experiment, colonic migrating motor complexes (CMMCs), smooth muscle spontaneous contractile experiments, intracellular electrical recordings, western blotting analysis, and quantitative polymerase chain reaction were applied in this study.RESULTS:
The mRNA and protein expressions of c-KIT and ANO1 channels were significantly decreased in the colons of DSS-colitis mice. The colonic artificial fecal-pellet transit experiment in vitro was significantly delayed in DSS-colitis mice. The CMMCs and smooth muscle spontaneous contractions were significantly decreased by 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), an ANO1 channel blocker, and NG-Nitro-L-arginine methyl ester hydrochloride (L-NAME), an inhibitor of nitric oxide synthase activity, in DSS-colitis mice compared with that of control mice. Intracellular electrical recordings showed that the amplitude of NPPB-induced hyperpolarization was more positive in DSS-colitis mice. The electric field stimulation-elicited nitric-dependent slow inhibitory junctional potentials were also more positive in DSS-colitis mice than those of control mice.CONCLUSION:
The results suggest that colonic transit disorder is mediated via downregulation of the nitric oxide/ICC/ANO1 signalling pathway in DSS-colitis mice.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Sodium
/
In Vitro Techniques
/
RNA, Messenger
/
Down-Regulation
/
Blotting, Western
/
Polymerase Chain Reaction
/
Myoelectric Complex, Migrating
/
Dextrans
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Colitis
/
Colon
Limits:
Animals
Language:
English
Journal:
Journal of Neurogastroenterology and Motility
Year:
2019
Document type:
Article