Inhibitory effect of endophytic fungi from Dysosma versipellis on HIV-1 IN-LEDGF/p75 interaction / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1808-1813, 2019.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-773163
Responsible library:
WPRO
ABSTRACT
To determine the inhibitory effect of endophytic fungi from Dysosma versipellis on HIV-1 IN-LEDGF/p75 interaction,the protein-protein interaction between human immunodeficiency virus type 1( HIV-1) integrase and lens epithelial growth factor p75 protein( LEDGF/p75) was used as a target. The homogeneous time-resolved fluorescence( HTRF) technique was used in the inhibitory activity assay. The results showed that eight endophytic fungi with anti-IN-LEDGF/p75 interaction activity were screened out from fifty-three strains with different morphological characteristic. Among them,106 strain showed strong inhibitory activity against HIV-1 IN-LEDGF/p75 interaction with IC50 value of 5. 23 mg·L-1,and was identified as a potential novel species of Magnaporthaceae family by the analyses of ITS-rDNA,LSU and RPB2 sequences data. This study demonstrated that potential natural active ingredients against the HIV-1 IN-LEDGF/p75 interaction exist in the endophytic fungi of D. versipellis. These results may provide available candidate strain resources for the research and development of new anti-acquired immunodeficiency syndrome drugs.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Chemistry
/
HIV-1
/
HIV Integrase
/
Berberidaceae
/
Intercellular Signaling Peptides and Proteins
/
Endophytes
/
Fungi
/
Metabolism
/
Microbiology
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2019
Document type:
Article