Inducing Effect of Decitabine on Apoptosis of KMS-18 Myeloma Cells and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1149-1153, 2019.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-775750
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of decitabine on proliferation and apoptosis of multiple myeloma KMS-18 cells and its possible mechanism.@*METHODS@#CCK-8 was used to detect cell proliferation, flow cytometry was used to detect the changes of apoptosis, real-time quantitative PCR was used to detect the expression of P53 gene mRNA in myeloma KMS-18 cells, and MSP assay was used to detect the methylation status of P53 gene promoter.@*RESULTS@#The proliferation inhibition and apoptosis of KMS-18 cells significantly increased after treatment by decitabine (P<0.05). The expression of P53 mRNA increased in KMS-18 cells after treatment of decitabine (P<0.05). The methylation status of the P53 gene promoter in KMS-18 cells could be partially reversed by decitabine.@*CONCLUSION@#Decitabine can inhibit the proliferation of KMS-18 cells and induce their apoptosis, its mechanism ralates with partially reversing the methylation of P53 gene promoter in KMS-18 cells.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Apoptosis
/
DNA Methylation
/
Cell Line, Tumor
/
Cell Proliferation
/
Decitabine
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2019
Document type:
Article