Increased Phosphorylation of PI3K/Akt/mTOR in the Obstructed Kidney of Rats with Unilateral Ureteral Obstruction / 전남의대학술지
Chonnam Medical Journal
; : 108-112, 2013.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-788276
Responsible library:
WPRO
ABSTRACT
The present study aimed to investigate changes in the mammalian target of rapamycin (mTOR) signaling pathway in the obstructed kidney of rats with unilateral ureteral obstruction (UUO). Male Sprague-Dawley rats were unilaterally obstructed by ligation of the left proximal ureter for 7 days. Control rats were treated in the same way except that no ligature was made. The expression levels of phosphorylated phosphatidylinositol 3-kinase (PI3K), Akt, and mTOR were determined in the kidney by semiquantitative immunoblotting. The protein expression levels of transforming growth factor (TGF)-beta1, Bax, and Bcl-2 were also determined in the kidney. The phosphorylation of PI3K, Akt, and mTOR was increased in the kidney of ureteral obstruction rats compared with the control. In the obstructed kidney, the protein expression of TGF-beta1 and Bax was also increased, whereas Bcl-2 expression was decreased. In conclusion, the phosphorylation of PI3K/Akt/mTOR was increased in the obstructed kidney of rats with UUO.
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Kidney, Renal Pelvis and Ureter Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Ureter
/
Ureteral Obstruction
/
Fibrosis
/
Immunoblotting
/
Transforming Growth Factors
/
Rats, Sprague-Dawley
/
Apoptosis
/
Sirolimus
/
Transforming Growth Factor beta1
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Chonnam Medical Journal
Year:
2013
Document type:
Article