Determination of content of cucurbitacin B in cucurbitacins by HPLC / 药学实践杂志

ABSTRACT

Objective To determina the content of cucurbitacin B in cucurbitacins .Methods The highly effective liquid phase chromatography was used,with Waters Symmetry C18(250 mm ×4.6 mm 5μm) column.The mobile phase was acetonitrile-wa-ter (51:49), the flow rate was 1 ml/min, column temperature was 25 ℃, the detection wavelength was 228 nm.Results Cucurb-itacin B regression equation:Y=1 067.3C-0.508 4 (r=0.999 9), the average recovery rate was 99.39%(n=6), RSD was 0.56%, which showed that this method had a good recovery rate .Conclusion The HPLC method for the determination of content of cucurbitacin B in cucurbitacins was simple , reproducible , accurate .