Research on fingerprint analysis of platycodonis liquid extract by UPLC-ELSD / 药学实践杂志

ABSTRACT

Objective To develop a stable method for fingerprint analysis of extractum platycodi liquidum to ensure its u‐niformity and stability .Methods UPLC‐ELSD was applied to obtain a chromatogram with 11 well separated peaks ,and the chromatographic condition was as followsmobile phase was gradient elution by acetonitrile‐water ,column was ACQUITY UPLC BEH C18 (2 .1 × 100 mm ,1 .7 μm) with the column temperature of 30 ℃ and the flow rate of 0 .2 ml/min .Results The chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM .Similar‐ities were all greater than 0 .90 ,which could meet the requirement for chromatographic fingerprint of TCM .Conclusion The stability ,repeatability and precision of the developed method were all validated ,and the chromatographic fingerprint analysis was a rapid and accurate method for the quality evaluation of traditional Chinese medicine .