Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra / 药学实践杂志

ABSTRACT

Objective To establish the HPLC fingerprint of Ginseng Radix et Rhizoma Rubra for its quality control . Methods Sharpsil-T C18 column(4 .6 mm × 250 mm ,5 μm)was used with acetonitrile-water in gradient elution mode .The flow rate was 1 .0 ml/min ,the column temperature was maintained at 30 ℃ ,and the detective wavelength was 203 nm .Results 24 co-possessing peaks were selected as fingerprint peaks and the similarities between each of the ten areas and the standard chro-matographic fingerprints of Panax ginseng Rubra were calculated while Rb1 peak selected as the reference peak .The similarity was more than 0 .940 .Eleven chemical compounds were identified by comparing the reference substance .Conclusion The method could be used for the quality control of Panax ginseng Rubra with good precision ,stability ,and reproducibility .