The methodology validation and content assay of total polysaccharides in Pudi Enema / 药学实践杂志
Journal of Pharmaceutical Practice
; (6): 248-251, 2017.
Article
in Zh
| WPRIM
| ID: wpr-790744
Responsible library:
WPRO
ABSTRACT
Objective To establish the assay method for the total polysaccharide in Pudi Enema.Methods Phenol-sulfuric acid method was used for chromogenic reaction.The content of total polysaccharide was measured by UV spectrophotometry at 488.8 nm.Results The total polysaccharides calibration curve was at the range of 0~22.635 mg/L, with regression function being Y=0.062 06 X-0.003 34(r=0.999 8).The recovery of calycosin was 98.36%(RSD=2.34%).Conclusion This method is sensitive,rapid,accurate and reliable.It can be used to assay the content of total polysaccharide in Pudi Enema.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Journal of Pharmaceutical Practice
Year:
2017
Document type:
Article