Survival rate of cryopreserved rat fat granuales after autologous transplantation / 中华医学美学美容杂志

ABSTRACT

Objective To observe the fat particles survival situations in autologous transplantation under different conditions of low temperature.Methods Three sample groups of adult Wistar rats were injected with fresh autologous fat particles,which were stored under 80 ℃ for two months and under-196 ℃ for two months respectively,and the volume,parallel histological detection,glucose transport measurement,MTT experiments and other data were compared and analyzed one month and two months after the transplantation.Results One month after transplantation,graft survival rate was higher in Group injected fresh autologous fat particles than that of Group injected autologous fat particles stored under-80 ℃ and Group injected autologous fat particles stored under -196 ℃ (P＜0.05);Two months after transplantation,the differences among three groups had no statistical significance.Before the transplantation,the activity of autologous fat particles stored in -80 ℃ and-196 ℃ were 68.7％ and 62.5％ of fresh autologous fat particles,respectively,two months after cryopreservation.One month after the transplantation,we found that the activities of autologous fat particles were higher in fresh group than that of-196 ℃ and-80 ℃,There was no significant difference between the groups stored in-196 ℃ and-80 ℃.Two months after the transplantation,the activities of autologous fat particles were higher in fresh group than that of-196 ℃ and -80 ℃.Before transplantation,the particles of fat histology showed no significant difference among three groups.One month after transplantation,fat vacuoles and distinct inflammatory infiltration were observed in all three groups,with the group stored in-80 ℃ as the most serious affected.Two months after transplantation,fresh fat particles and Group stored in-196 ℃ showed large quantity of tightly packed intact fat cells,scattered around the central area in fat vacuoles,while Group stored in -80 ℃ still had a large quantity of fat vacuoles,but the fat vacuoles were obviously fused.Conclusions The fat stored at-80 ℃ and-196 ℃ could meet the needs of clinical use in the short time.The fat stored at-196 ℃ shows the best activity.The fat particles stored at-196 ℃ could be used to establish better blood circulation.The heaviest inflammatory reaction occurs in the graft zone one month after fat transplantation and the decrease of transplanted particle activity is the most obvious.